Additional Selective and Differential Cell Culture Media
Bioworld DMEM/Ham's F-12 (1:1) w/ HEPES, 1 L
Dublecco's Modified Eagles Medium/Nutrient Mixture Ham's F-12 (DMEM/Ham's F-12) was created after extensive study of Harry Eagle's first reports. Since then, studies have been underway to determine the media requirements for individual cell types. The medium found to be most satisfactory for these studies was a 1:1 mixture of Dublecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. To help compensate for the buffering capacity lost with the removal of serum, HEPES has been added in some modifications at a final concentration of 15mM. DMEM/Ham's F-12 (1:1 Mixture, powder), item #785060, contains L-glutamine, pyridoxine hydrochloride and 15mM HEPES buffer. Powder does not include sodium bicarbonate. Click here to see a DMEM/Ham's F12 1:1 Medium Components Table. Click here to see a Sodium Bicarbonate addition table for powdered media.
Bioworld Fast Terrestrial Orchid Medium, 50 L
Fast Terrestrial Orchid MediumContains Macronutrients, Micronutrients, Vitamins, Sucrose, Fructose, Agar, and Supplements required to culture terrestrial orchidsCytokines and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking Concentration: 27.84 g/LpH: 5.2-6.0Partially soluble in water
Bioworld Murashige and Skoog (MS) Medium, Buffered, 50 L
Murashige & Skoog (MS) MediumContains Macronutrients and Micronutrients as described by Murashige & Skoog (1967)Buffered with MES MES is an excellent buffer for plant media because of high buffer capacity and its pH range of 5.5-6.7 Sucrose, Cytokines, Vitamins, and Auxins should be added as needed Working Concentration: 4.83 g/LProvided as 1 unit to make 10L, 25L, or 50L of mediumPlant tissue culture tested
Bioworld DMEM, High Glucose with L-glut & Pyridoxine HCL, w/o Sodium phosphate and Sodium pyruvate.(powder), 10 L
Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME), containing four-fold concentrations of the amino acids and vitamins found in BME, as well as additional supplementary components. The original formulation of DMEM contained 1000mg/L of glucose, but since has been raised in some modifications to 4500mg/L, giving the terms of Low Glucose or High Glucose. Several other modifications over the years, in addition to the alternative glucose levels, have broadened the spectrum of cell types supported by DMEM. DMEM (powder), High glucose with L-glutamine contains a pyridoxine hydrochloride supplement. DMEM does not include sodium phosphate, sodium bicarbonate or sodium pyruvate.
Bioworld Murashige and Skoog (MS) Medium, Buffered, 25 L
Murashige & Skoog (MS) MediumContains Macronutrients and Micronutrients as described by Murashige & Skoog (1967)Buffered with MES MES is an excellent buffer for plant media because of high buffer capacity and its pH range of 5.5-6.7 Sucrose, Cytokines, Vitamins, and Auxins should be added as needed Working Concentration: 4.83 g/LProvided as 1 unit to make 10L, 25L, or 50L of mediumPlant tissue culture tested
FUJIFILM BIOSCIENCES INC PRIME-XV T Cell CDM, 1 L
PRIME-XV T Cell CDM is the first commercially-available, chemically defined, animal component-free medium for the expansion and transduction of human T cells. The formulation is optimized to deliver consistently vigorous growth while maintaining T cell functionality and potency.Supports T cell expansion in static and dynamic automation systems while maintaining functionalityEliminates the adverse effects undefined components cause on T cell phenotypesSupports polarization to targeted T cell types, such as Th1, Th2, cytotoxic T cells, to further possible therapy applicationsPerforms transduction of T cells without requiring spinoculation or transduction enhancers
Bioworld Iscove's DMEM w/ L-Glutamine and 25 Mm HEPES, 1 L
Guilbert and Iscove published their modifications to Dulbecco's Modified Eagle's Medium (DMEM) in 1976. Their modification included the supplements of selenium, additional vitamins and amino acids, sodium pyruvate and HEPES buffer. In addition, ferric nitrate was replaced with potassium nitrate. When further supplemented with albumin, lecithin and transferrin, this medium supported the growth of precursor cell of macrophages and erythocytes without serum supplementation. Iscove's Medium has since been shown to support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes and a variety of hybrid cells. Iscove's Modified Dulbecco's Media (DMEM) liquid, item #785105, contains l-glutamine and 25mM HEPES buffer. Click here to see an Iscove's Modified Dulbecco's Medium Components Table.
Sigma Aldrich Fine Chemicals Biosciences MINIMUM ESSENTIAL MEDIUM EAGLE
NC3453419 MINIMUM ESSENTIAL MEDIUM EAGLE
Sigma Aldrich Fine Chemicals Biosciences GLYCEROL DIMETHACRYLATE 500ML
NC3594318 GLYCEROL DIMETHACRYLATE 500ML
Bioworld DMEM, High Gluc w/L-Glut, Pyridoxine HCl and Sodium Pyruvate, 1 L
DMEM (powder), High glucose with L-glutamine, item #785017, contains a pyridoxine hydrochloride supplement and 110 mg/L sodium pyruvate.Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME), containing four-fold concentrations of the amino acids and vitamins found in BME, as well as additional supplementary components. The original formulation of DMEM contained 1000mg/L of glucose, but since has been raised in some modifications to 4500mg/L, giving the terms of Low Glucose or High Glucose. Several other modifications over the years, in addition to the alternative glucose levels, have broadened the spectrum of cell types supported by DMEM.
Bioworld McCoy's 5A Medium w/o L-Glut, 1 L
McCoy's 5A Media (modified) was originally developed by McCoy and his co-workers in 1959 and was known then as Basal Medium 5A. McCoy published the research he had conducted regarding the Basal Medium 5A, which detailed the amino acid requirements for the culture of Novikoff Hepatoma Cells. Basal Medium 5A was subsequently modified resulting in a similar media known as McCoy's 5A Media. Iwakata and Grace further modified the medium for use in work with human myeloblasts. McCoy's 5A liquid media, item #785122, does not contain L-glutamine. Click here to see a McCoy's 5A Media Components Table.